Protein Complex Stoichiometry
We offer custom-made QconCATs and in-house developed comprehensive LC-MS/MS and data analysis workflows for:
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Analysis of protein complex components in whole cell lysates or after immunoprecipitation.
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Determination of subunit stoichiometry
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Quantitative measurement of protein complex subunits.
More information
The function, activity and location of protein complexes can be regulated via post-translational modification of key subunits but also through composition of its subunits. Qualitative and quantitative analysis of protein complexes using mass spectrometry is independent of antibodies and enables further insight into the regulation of protein complexes, metabolic pathways and signalling cascades in their native environment. Our QconCAT reference standards allow absolute quantification of individual protein subunits and to determine the stoichiometry of the complex subunits. Benefit from our profound expertise in dealing with comprehensive protein quantification projects. Receive support and assistance at every step of the project, from protocol development, peptide selection to measurement, data analysis and comprehensive reporting.
We quantify your proteins
Determine the stoichiometry of protein complex subunits in a single mass spectrometry experiment,
Individual service
Receive customized proteomics services with clear timelines, milestones, deliverables and detailed reporting.
Support
Our experienced team provides consulting and support at every stage of the project.
Scalable
From exploratory studies to routine measurements and high throughput analyses.
High sensitivity
Stable, accurate and ultra-sensitive protein analysis with high-end instruments
Antibody-free
Fast method setup by unbiased, antibody-independent protein detection.
Quantitative
Determine the stoichiometry of protein complex subunits using our proprietary QconCAT reference standards.
Contact us now:
Discuss your requirements with our experienced professionals.
E-Mail: info[at]polyquant.com
Phone: +49 (0)9405 96999 10
Fax: +49 (0)9405 96999 28
References
CRAF dimerization with ARAF regulates KRAS-driven tumor growth
Venkatanarayan A, et al.,
Cell Rep. 2022 Feb 8;38(6):110351. [PubMed]
Venkatanarayan et al. used quantitative proteomics to demonstrate increased levels of CRAF:ARAF dimers in KRAS mutant cells and PRM analysis to quantify the stoichiometric relationships between wt and mutant MAPK components using a QconCAT incorporating wild type and mutant sequences for detection of disease associated mutations.
PIKES Analysis Reveals Response to Degraders and Key Regulatory Mechanisms of the CRL4 Network
Reichermeier KM, et al.,
Mol Cell. 2020 Jan 15. [Pubmed]
Reichermeier et al. determined the stoichiometric relationships between approx. 30 proteins in cell lysates and immunoprecipitated samples using targeted proteomics and a QconCAT comprising of 67 peptides for 31 proteins.
Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach
Stelder SK, et al.,
J Proteome Res. 2018 Feb 2;17(2):903-917 [Pubmed]
Stelder et al. quantified crucial spore proteins using a QconCAT reference standard, determining the absolute abundance of 21 proteins, covering approx. 5.66% of the total spore weight in wild type and approx. 4.13% in the ΔCotE mutant.
Rigorous determination of the stoichiometry of protein phosphorylation using mass spectrometry
Johnson H, et al.,
Journal of the American Society for Mass Spectrometry 2009 Dec;20(12):2211-20. [PubMed]
Johnson et al. use QconCATs to determine absolute protein concentrations and the stoichiometry of phosphorylation at predefined sites.