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Phage analytics

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We support phage research and development of phage-based therapeutics:

  • Quantification of phage proteins

  • Determination of virion stoichiometry

  • Quality control of therapeutics (HCP detection and quantification)

  • Analyses and support from early R&D to routine testing

  • GMP-conform procedures

More information

Antimicrobial resistance has increased over the past decades and the reducing efficiency of antibiotics demands for new treatments. Phages are natural antibacterial agents and phage research has received a lot of attention, especially its therapeutic application. As for other biologically derived therapeutics phage-based products demand for sophisticated quality control. Mass spectrometry enables the identification of thousands of proteins in one measurement, enabling the unbiased detection of host contaminant proteins in therapeutic phages and viral vaccines at low setup costs. Our proprietary QconCAT technology allows for quantitative determination of phage proteins and the stoichiometry of virions, measuring up to 50 virus proteins with a single QconCAT reference standard. For example, covering the proteome of phiKZ would demand for 4-6 QconCATs, saving you time preparing complex peptide mixes. Additionally, there is no need for production of specific antibodies, enabling fast assay development. Further reading: Applications of targeted proteomics in systems biology and translational medicine Ebhardt et al. Proteomics. 2015 Sep;15(18):3193-208

Phage Analytics Services

Quantitative Proteomics

Established workflows and supporting bioinformatics for e. g.

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  • Quantification of phage proteins

  • Time-resolved protein expression of viral proteins and/or selected host proteins

  • Stoichiometry of whole phages

Host Cell Protein Analytics

Fast and robust measurements for routine analyses. We provide GMP-conform/GMP-approved procedures for:

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  • Highly sensitive detection of HCPs

  • Highly accurate quantification of selected HCPs

Custom Service

Customized solutions, tailored for your research project.

From consulting to protocol development and comprehensive analysis, performed and evaluated by experiences professionals.

Receive support throughout the project and complete reporting.

Antibody-free phage analysis

Mass spectrometry analyses enable quantitative analyses of phage subunits as well as quality control of therapeutic phage preparations.

Individual service and support

Receive customized proteomics services and detailed reporting.

Our experienced team provides consulting and support at every stage of the project.

Cost-efficient

Precise quantification at low per-sample cost using our proprietary QconCAT technology.

Scalable

Fast and robust measurements.

From exploratory studies to routine measurements and high throughput analyses.

High sensitivity

Stable, accurate and ultra-sensitive protein analysis with high-end instruments

Antibody-free

Fast method setup by unbiased, antibody-independent protein detection.

Efficient

Measurement in complex matrices

(multiple phages, disturbing chemicals, phage proteins in host cells)

Contact us now:

Discuss your requirements with our experienced professionals.

E-Mail: info[at]polyquant.com
Phone: +49 (0)9405 96999 10
Fax: +49 (0)9405 96999 28

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