Metaproteomics
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Determine the biodiversity of microbial or viral communities and multi-organism systems
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Identify and quantify proteins from microbial communities
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Analyze the expressed proteins for each organism
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Quantification of per species biomass
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Track substrate metabolisms
More information
Metaproteomics is a rapidly growing research area, addressing the microbial composition of environmental samples (soil, feces, sewage, water etc). We have developed metaproteomics workflows covering protein extraction, peptide separation, LC-MS/MS measurements and data analysis. Our techniques allow us to determine the organisms present in a sample and their proportions as well as comprehensive analysis of the expressed proteins for each organism. Further reading: Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities mSystems. 2019 May 21;4(3):e00115-19.
Metaproteomics Service
Workflow
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Example: Analysis of sewage sludge of unknown composition
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A protein sample of sewage sludge of unknown composition was solubilized and 30 µg of protein were digested with trypsin. The peptides were then fractionated by binding the peptides to a filter, followed by elution with increasing concentrations of acetonitrile (ACN). The samples were measured on an Orbitrap HF-X mass spectrometer coupled to an AQUITY UPLC system, using a 25 cm M‑Class column. Gradients of 60, 130 or 300 min were used to separate the peptides. Peptides were identified using a de novo peptide sequencing algorithm and by a sectioning database approach, searching against the whole Uniprot bacteria database. The organisms identified in the first round of analysis were used to construct specific databases for more in-depth analysis of the provided sample.
Detailed analysis of highly complex samples
Microbial communities are highly complex samples and therefore difficult to analyze. We have established bioinformatics analyses, sample preparation techniques and LC-MS/MS workflows for various metaproteomics projects.
Individual service
Receive customized metaproteomics services with clear timelines, milestones, deliverables and detailed reporting.
Support
Our experienced team provides consulting and support at every stage of the project.
Established workflows
Established sample preparation protocols for LC-MS/MS measurement in complex matrices.
Data analysis
Data evaluation by automated and manually curated data analysis workflows.
High sensitivity
Stable, accurate and ultra-sensitive protein analysis with high-end instruments
Antibody-free
Antibody-free protein detection and quantification.