Isoform-specific Protein Quantification
Protein isoforms are difficult to distinguish using commercially available antibodies.
By detecting isoform-specific unique peptides, mass spectrometry enables differentiation between wild type, mutants and splice variants without the need for isoform-specific antibodies.
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We offer in-house developed comprehensive data analysis workflows for isoform-specific protein detection. Combined with a well designed QconCAT reference standard, the analysis can be expanded to provide you with quantitative data for highly similar protein isoforms.
Isoform-specific protein quantification
Workflow
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Peptide selection and production of QconCAT reference standard
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Sample preparation
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Mix QconCAT and sample
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Trypsin digestion
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Mass spectrometry analysis
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Data analysis and reporting
Workflow for isoform-specific protein quantification using LC-MS/MS and QconCATs.
A sample containing proteins of interest is digested with an appropriate protease. After LC separation, the peptides are detected using high resolution mass spectrometry. Quantities of the target proteins are calculated based on the signal detected for the isotope-labeled reference peptides.
Example
Absolute quantification of protein expression of a wild type protein (WT) and isoforms (V1, V2) at different time points after treatment of the host organism.
Distinguish highly homologous proteins
Build on our in-house developed bioinformatics analysis for selection of unique peptides, enabling isoform specific protein detection through established LC-MS/MS workflows.
Individual service
Receive customized proteomics services with clear timelines, milestones, deliverables and detailed reporting.
Support
Our experienced team provides consulting and support at every stage of the project.
Scalable
From exploratory studies to routine measurements and high throughput analyses.
High sensitivity
Stable, accurate and ultra-sensitive protein analysis with high-end instruments
Antibody-free
Fast method setup by unbiased, antibody-independent protein detection.
Quantitative
Receive absolute protein quantities with our proprietary QconCAT technology.